What is the difference between MEM and DMEM?
What is the difference between MEM and DMEM?
anyway , DMEM is basically MEM with additional amino acids , vitamins and proteins to prevent the adhesion of the cells to the sides of the flask . DMEM is richer in a variety of nutrients than MEM and is a very good choice for many cell types. Cells will grow faster and deplete the medium slower in DMEM.
How much glucose is in high glucose DMEM?
Specifications
| Glucose Concentration: | 4500 mg/L |
|---|---|
| Volume (Metric): | 500 ml |
| Product Line: | Gibco™ |
| Form: | Liquid |
| Glutamine: | L-Glutamine |
What is high glucose DMEM?
DMEM (Dulbecco’s Modified Eagle Medium) is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. DMEM is well suited for supporting the growth of a broad spectrum of mammalian cell lines.
Is MEM same as EMEM?
MEM Spinner was developed when calcium component was removed from the MEM medium, and resulted in a medium that supports the growth of cells in suspension. Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture.
Does RPMI 1640 contain glucose?
One liter of RPMI 1640 contains: Glucose (2 g) pH indicator (phenol red, 5 mg) Salts (6 g sodium chloride, 2 g sodium bicarbonate, 1.512 g disodium phosphate, 400 mg potassium chloride, 100 mg magnesium sulfate, and 100 mg calcium nitrate)
How much glucose do you add to the media?
The amount of glucose in cell culture formulations ranges from 1 g/L (5.5 mM) to as high as 10 g/L (55 mM). Many classical media are supplemented with approximately 5.5 mM D-glucose which approximates normal blood sugar levels in vivo.
What is the pH of DMEM?
DMEM is buffered for high CO2. Your DMEM probably has 3.7 g sodium bicarbonate which will provide a pH of 7.4 in 10% CO2. If you remove the high CO2, the sodium bicarbonate takes over and you get a basic pH.